Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Proline Uptake and Utilization by Chlorella pyrenoidosa.

A D McNamer1, C R Stewart

  • 1Department of Botany and Plant Pathology, Iowa State University, Ames, Iowa 50010.

Plant Physiology
|December 1, 1973
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Knowledge of Down syndrome in pregnant women from different ethnic groups.

Prenatal diagnosis·2001
Same author

The effects of magnesium supplementation on exercise performance.

Medicine and science in sports and exercise·2001
Same author

Genes and regulatory sites of the "host-takeover module" in the terminal redundancy of Bacillus subtilis bacteriophage SPO1.

Virology·1998
Same author

A comparative study of women with chronic pelvic pain, chronic nonpelvic pain and those with no history of pain attending general practitioners.

British journal of obstetrics and gynaecology·1998
Same author

Genes that protect against the host-killing activity of the E3 protein of Bacillus subtilis bacteriophage SPO1.

Journal of bacteriology·1995
Same author

Infertility, obstetric and gynaecological problems in coeliac sprue.

Digestive diseases (Basel, Switzerland)·1994

Chlorella pyrenoidosa utilizes proline, an amino acid, for growth and energy. Proline uptake depends on glucose availability and intracellular carbohydrate levels, influencing its metabolism via protein incorporation or Krebs cycle pathways.

Area of Science:

  • * Cellular and Molecular Biology
  • * Algal Physiology
  • * Biochemistry

Background:

  • * Understanding nutrient uptake and utilization is crucial for algal biotechnology.
  • * Proline, an amino acid, serves various roles in plant and algal metabolism.
  • * Chlorella pyrenoidosa is a widely studied freshwater green alga.

Purpose of the Study:

  • * To elucidate the conditions governing proline uptake by Chlorella pyrenoidosa.
  • * To investigate the metabolic fate of proline within the algal cells.
  • * To determine the influence of glucose and growth phase on proline assimilation.

Main Methods:

  • * Cultivation of Chlorella pyrenoidosa under varying conditions (growth phase, glucose presence/absence).
  • * Measurement of proline uptake rates.

Related Experiment Videos

  • * Analysis of proline metabolism through incorporation into proteins and CO(2) oxidation.
  • * Tracing metabolic pathways via conversion to glutamic acid and the Krebs cycle.

    • Main Results:

    • * Proline uptake occurs in late log phase after glucose depletion in growing cultures.
    • * Stationary phase cultures require glucose for proline uptake.
    • * Carbohydrate availability is essential for proline uptake, but intracellular accumulation inhibits it.
    • * Proline is metabolized by incorporation into proteins and oxidation to CO(2) via glutamic acid and the Krebs cycle.

    Conclusions:

    • * Proline uptake in Chlorella pyrenoidosa is a regulated process influenced by growth phase and carbohydrate status.
    • * A balance of external carbohydrates is necessary for efficient proline uptake, preventing self-inhibition.
    • * Proline serves as a carbon and nitrogen source, integrated into cellular components and energy pathways.