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Deoxyribonucleic Acid polymerase from wheat embryos.

Y Y Mory1, D Chen, S Sarid

  • 1Department of Biophysics, The Weizmann Institute of Science, Rehovot, Israel.

Plant Physiology
|March 1, 1974
PubMed
Summary
This summary is machine-generated.

Researchers identified a DNA polymerase in wheat embryos that synthesizes new DNA strands. This enzyme, in vitro, uses a DNA template to create complementary DNA chains, indicating a semiconservative DNA replication mechanism.

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Area of Science:

  • Molecular Biology
  • Biochemistry
  • Plant Science

Background:

  • DNA replication is a fundamental biological process essential for cell division and heredity.
  • Understanding the enzymes involved in DNA synthesis is crucial for deciphering genetic mechanisms.
  • Plant embryos represent a rich source of enzymes involved in nucleic acid metabolism.

Purpose of the Study:

  • To isolate and characterize a DNA-dependent DNA polymerase from wheat embryos.
  • To investigate the requirements and characteristics of in vitro DNA synthesis catalyzed by this enzyme.
  • To determine if the observed DNA synthesis mechanism is consistent with semiconservative replication.

Main Methods:

  • Extraction and purification of a soluble DNA-dependent DNA polymerase from wheat embryos.
  • In vitro assays measuring the incorporation of radioactive thymidine triphosphate into DNA.
  • Analysis of the synthesized DNA product, including chain length, buoyant density, and template complex formation.

Main Results:

  • A soluble DNA-dependent DNA polymerase was successfully extracted from wheat embryos.
  • Enzymatic activity required the enzyme, all four deoxyribonucleotide triphosphates, Mg(2+), and a DNA template.
  • Synthesis occurred on various DNA forms (native, denatured, double-stranded) producing shorter polydeoxynucleotides that formed double-stranded complexes with the template.

Conclusions:

  • The wheat embryo DNA polymerase catalyzes DNA synthesis dependent on a DNA template.
  • The characteristics of the synthesized DNA product suggest a semiconservative mode of DNA replication.
  • This study identifies a key enzyme involved in plant DNA replication and provides evidence for its mechanism.