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Homoserine esterification in green plants.

J Giovanelli1, S H Mudd, A H Datko

  • 1Laboratory of General and Comparative Biochemistry, National Institute of Mental Health, Bethesda, Maryland 20014.

Plant Physiology
|November 1, 1974
PubMed
Summary

Plant extracts were surveyed for synthesizing homoserine esters, potential precursors for methionine and threonine. Only Pisum sativum and Lathyrus sativus synthesized O-acylhomoserine esters, while O-phosphohomoserine was synthesized by all plants examined.

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Area of Science:

  • Biochemistry
  • Plant Physiology
  • Metabolic Pathways

Background:

  • Homoserine esters are potential precursors for methionine and threonine synthesis in plants.
  • Diverse plant species were investigated for their capacity to synthesize various homoserine esters.

Purpose of the Study:

  • To survey phylogenetically diverse plants for the synthesis of specific homoserine esters.
  • To identify the major activated homoserine derivative in plants.

Main Methods:

  • Surveying plant extracts for the ability to synthesize O-acetyl-, O-oxalyl-, O-succinyl-, O-malonyl-, and O-phosphohomoserine.
  • Analyzing the specific synthesis of O-acylhomoserine esters from homoserine and corresponding acyl-CoA derivatives.
  • Detecting O-phosphohomoserine synthesis via homoserine kinase activity.

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Main Results:

  • O-acylhomoserine ester synthesis was exclusively detected in Pisum sativum (O-acetylhomoserine) and Lathyrus sativus (O-oxalylhomoserine).
  • All examined plant extracts, including legumes, algae, horsetails, gymnosperms, and angiosperms, demonstrated O-phosphohomoserine synthesis.
  • O-phosphohomoserine was identified as the predominant activated homoserine derivative among the five esters studied.

Conclusions:

  • O-phosphohomoserine is the major activated homoserine derivative in plants.
  • The synthesis of O-acylhomoserine esters is limited to specific plant species.
  • These findings support the role of O-phosphohomoserine in methionine biosynthesis pathways.