Related Experiment Video
Updated: Aug 8, 2026

Cycloheximide Chase Analysis of Protein Degradation in Saccharomyces cerevisiae
Published on: April 18, 2016
Mechanism of sulfate transport inhibition by cycloheximide in plant tissues
Abstract:
Inhibition by cycloheximide of sulfate transport in both barley roots (Hordeum vulgare L.) and potato tuber (Solanum tuberosum L.) increases with increasing inhibitor concentration only to a limited extent, depending on the length of the tissue incubation with the inhibitor. In contrast to this, increasing concentrations of dinitrophenol have a rapid and total inhibitory effect on the active transport. Leucine transport in the same tissues is strongly inhibited by dinitrophenol but is not affected by cycloheximide, whereas incorporation into protein is mainly inhibited by cycloheximide. It appears that the mechanism of transport inhibition by cycloheximide in plant tissues consists in stopping new carrier synthesis and not in the disruption of energy flow. Sulfate carriers show comparable decay rates in barley roots and potato tuber, the mean life being shorter than that of the leucine carriers. These appear more stable in roots than in storage tissues.
Related Concept Videos
Sulfur Assimilation
Microbes and the Sulfur Cycle
Riboswitches
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
Responses to Salt Stress
Microbe-Plant Interactions
Drugs that Destabilize Microtubules

