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Related Experiment Videos

Bound auxin formation in growing stems.

P J Davies1

  • 1Section of Genetics Development and Physiology, Division of Biological Sciences, Cornell University, Ithaca, New York 14850.

Plant Physiology
|February 1, 1976
PubMed
Summary
This summary is machine-generated.

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Plants form "bound auxin" conjugates rapidly after indoleacetic acid (IAA) application. This process is energy-dependent and involves RNA and protein synthesis, suggesting an early role for auxin in regulating its own metabolism.

Area of Science:

  • Plant Physiology
  • Biochemistry
  • Molecular Biology

Background:

  • Auxin, a key plant hormone, exists in free and conjugated forms.
  • Bound auxin, insoluble in organic solvents but releasable by hydrolysis, represents a significant metabolic pool.
  • Understanding bound auxin formation is crucial for elucidating auxin homeostasis and signaling.

Purpose of the Study:

  • To characterize the formation and properties of bound auxin in pea stems.
  • To investigate the metabolic requirements and regulatory mechanisms of bound auxin synthesis.
  • To explore the potential physiological significance of bound auxin.

Main Methods:

  • Fractionation of pea stem extracts into water-soluble, water-insoluble/NaOH-hydrolyzable, and insoluble bound auxin.

Related Experiment Videos

  • Quantification of bound auxin formation following exogenous application of labeled indoleacetic acid (IAA).
  • Assessment of the effects of metabolic inhibitors (KCN, 6-methylpurine, cycloheximide, deoxyglucose) and aging on bound auxin synthesis.

    • Main Results:

    • Bound auxin formation initiated within 15 minutes of IAA application and increased over 6 hours.
    • Light-grown stems produced more bound auxin than dark-grown stems.
    • Bound auxin synthesis was dependent on respiratory metabolism and required RNA and protein synthesis, with an early inductive effect of IAA.

    Conclusions:

    • Bound auxin is a significant and rapidly formed product of auxin metabolism in plants.
    • Its formation is an active, energy-dependent process regulated early in the auxin response pathway.
    • The precise physiological role of bound auxin remains to be determined but is likely important in plant growth.