Related Experiment Video
Updated: Aug 8, 2026

Isotopic Effect in Double Proton Transfer Process of Porphycene Investigated by Enhanced QM/MM Method
Published on: July 19, 2019
Isotope Discrimination by Ribulose 1,5-Diphosphate Carboxylase: No Effect of Temperature or HCO(3) Concentration
1Plant Physiology Division, Department of Scientific and Industrial Research, Palmerston North, New Zealand.
Abstract:
Carbon 13 isotope discrimination by ribulose 1,5-diphosphate carboxylase from soybean (Glycine max [Merr.] cv. Amsoy) was studied as a function of temperature, bicarbonate concentration, and pH. None of these factors affected the degree of discrimination against (13)C. The average delta(13)C was -28.3%, a value close to that found for whole C(3) plants. The zero temperature response observed here with ribulose 1,5-diphosphate carboxylase corroborates data from whole plants. The lack of effect of bicarbonate concentration on discrimination is consistent with both current theories of alternate forms of carboxylase.
More Related Videos
Related Concept Videos
Riboswitches
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
The Calvin Benson Cycle
Mass Spectrometry: Isotope Effect
Effects of Temperature on Free Energy
¹H NMR of Conformationally Flexible Molecules: Variable-Temperature NMR

