Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Leaf cell water and enzyme activity.

S M Arad1, A E Richmond

  • 1Department of Biology and the Research and Development Authority, Ben-Gurion University of the Negev, Beer-Sheva, Israel.

Plant Physiology
|April 1, 1976
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Abscisic acid and the after-effect of stress in tobacco plants.

Planta·2014
Same author

Soluble polysaccharide and biomass of red microalga Porphyridium sp. alter intestinal morphology and reduce serum cholesterol in rats.

The British journal of nutrition·2000
Same author

Structure of 3-O-(alpha-D-glucopyranosyluronic acid)-L-galactopyranose, an aldobiouronic acid isolated from the polysaccharides of various unicellular red algae.

Carbohydrate research·1990
Same author

Acetohydroxy Acid Synthase Activity in Chlorella emersonii under Auto- and Heterotrophic Growth Conditions.

Plant physiology·1990
Same author

Relationship between the Unicellular Red Alga Porphyridium sp. and Its Predator, the Dinoflagellate Gymnodinium sp.

Applied and environmental microbiology·1989
Same author

Effect of Nitrogen on Polysaccharide Production in a Porphyridium sp.

Applied and environmental microbiology·1988
Same journal

Autoinhibition of Plasma Membrane H+-ATPase1 Regulates Systemic Herbivore Defense in Arabidopsis.

Plant physiology·2026
Same journal

A deep learning model captures position-specific preferences of plant regulatory sequences and suggests genes under complex regulation.

Plant physiology·2026
Same journal

Nissolia brasiliensis as a non-nodulating model legume.

Plant physiology·2026
Same journal

Auxin response factor OsARF22 controls rice seed vigor by suppressing ABA signaling.

Plant physiology·2026
Same journal

The primary nitrate response TGA1 and TGA4 transcription factors are negative regulators of sulfate uptake and metabolism.

Plant physiology·2026
Same journal

TaSPL14-D diverged from its ortholog to regulate tiller angle in rice: a caveat for orthology-based functional inference.

Plant physiology·2026
See all related articles

Water stress in barley plants directly impacts enzyme activity, overriding hormonal effects. Increased water saturation deficit (WSD) elevates RNase activity, highlighting cell water content

Area of Science:

  • Plant Physiology
  • Biochemistry
  • Molecular Biology

Background:

  • Hormonal regulation typically controls enzyme activity in plants.
  • Water stress is a significant environmental factor affecting plant metabolism.
  • The role of cell water content in regulating enzyme activity under stress requires further elucidation.

Purpose of the Study:

  • To investigate the role of cell water content versus hormonal regulation in enzyme activity under water stress.
  • To determine the effect of salt-induced water stress on RNase activity in barley leaves.
  • To examine the influence of kinetin and abscisic acid on RNase activity and water saturation deficit (WSD) in salinated barley plants.

Main Methods:

  • Barley plants (Hordeum vulgare L.) were subjected to NaCl treatment to induce water stress.

Related Experiment Videos

  • Leaf water saturation deficit (WSD) was measured.
  • Leaf RNase and protease activities were quantified.
  • Hormonal treatments (kinetin, abscisic acid) were applied to salinated plants.
  • High relative humidity was used to alleviate water stress.

    • Main Results:

    • NaCl addition increased leaf RNase activity and leaf WSD in barley.
    • Kinetin and abscisic acid modified RNase activity and WSD, inverting typical hormonal effects.
    • A strong correlation was observed between enzyme activity (RNase, protease) and leaf WSD, independent of hormonal treatments.
    • Alleviating salt-induced water stress with high humidity prevented the rise in RNase activity.

    Conclusions:

    • Cell water content, indicated by WSD, plays a crucial regulatory role in enzyme activity under water stress, potentially superseding hormonal control.
    • RNase activity in barley leaves is closely linked to the level of water saturation deficit.
    • Hormonal effects on RNase activity and chlorophyll content can be altered by the prevailing water status of the plant.