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Starch degradation in the cotyledons of germinating lentils.

J F Tárrago1, G Nicolás

  • 1Departamento de Fisiología Vegetal, Facultad de Farmacia, Universidad de Santiago de Compostela, Spain.

Plant Physiology
|November 1, 1976
PubMed
Summary

During lentil germination, starch breakdown is initially slow, with phosphorylase activity peaking on day 3. Rapid starch depletion begins on day 4, coinciding with increased alpha-amylase activity.

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Area of Science:

  • Plant Biochemistry
  • Molecular Biology
  • Enzymology

Background:

  • Starch serves as a crucial energy reserve in plant seeds.
  • Understanding starch degradation during germination is key to plant physiology.

Purpose of the Study:

  • To investigate the enzymatic activities involved in starch breakdown in lentil cotyledons during early germination.
  • To characterize the types and roles of amylolytic enzymes and phosphorylase in this process.

Main Methods:

  • Assay of total amylolytic and phosphorylase activities in lentil cotyledons.
  • Polyacrylamide gel electrophoresis (PAGE) to separate and identify starch-degrading enzymes.
  • Heat treatment to differentiate enzyme properties.

Main Results:

  • Amylolytic activity is primarily attributed to alpha-amylase.
  • Phosphorylase activity increases, peaking on the third day of germination.
  • A significant increase in alpha-amylase activity on the fourth day correlates with rapid starch depletion.
  • PAGE revealed one phosphorylase, three alpha-amylases, and one beta-amylase.
  • Two distinct sets of alpha-amylase, differing in heat stability, were identified.

Conclusions:

  • Lentil seed germination involves a coordinated action of phosphorylase and alpha-amylase in starch degradation.
  • Alpha-amylase plays a dominant role in the later stages of early germination.
  • The presence of multiple alpha-amylase forms suggests complex regulatory mechanisms.