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Related Experiment Videos

Asparagine synthetase in corn roots.

I Stulen1, A Oaks

  • 1Department of Biology, McMaster University, Hamilton, Ontario, Canada L8S 4K1.

Plant Physiology
|November 1, 1977
PubMed
Summary

Asparagine synthetase levels differ between corn root tips and mature sections. Excising root tips increases enzyme synthesis, requiring RNA and protein production, unlike mature sections where activity declines.

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Area of Science:

  • Plant Biochemistry
  • Molecular Biology
  • Agricultural Science

Background:

  • Asparagine synthetase (AS) is a key enzyme in nitrogen metabolism.
  • Understanding AS regulation in plants is crucial for crop development.

Purpose of the Study:

  • To investigate the regulation of asparagine synthetase in different zones of corn roots.
  • To determine the role of RNA and protein synthesis in AS activity.

Main Methods:

  • Enzyme assays on corn (Zea mays) root tips and mature root sections.
  • Treatment with inhibitors of RNA (cordycepin, 6-methylpurine) and protein synthesis (cycloheximide).
  • Assessment of effects of azaserine and glucose on enzyme formation.

Main Results:

  • Asparagine synthetase levels are low in root tips and high in mature root sections.
  • Excising root tips induces a significant increase in AS activity, dependent on RNA and protein synthesis.
  • Mature root sections show a decline in AS activity, unaffected by inhibitors or glucose/asparagine.

Conclusions:

  • Asparagine synthetase synthesis in corn root tips is a de novo process regulated by RNA and protein synthesis.
  • Nitrogen assimilation and enzyme regulation differ significantly between young and mature root tissues in corn.

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