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Indoleacetaldehyde in cucumber seedlings.

W K Purves¹, H M Brown

¹Biochemistry and Biophysics Section, Biological Sciences Group, University of Connecticut, Storrs, Connecticut 06268.

Plant Physiology

|January 1, 1978

Summary

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Indoleacetaldehyde was identified in cucumber cotyledons using multiple analytical methods. Cucumber seedlings also metabolize this compound into indoleethanol.

Area of Science:

Plant biochemistry
Cucumber (Cucumis sativus L.) physiology

Background:

Indoleacetaldehyde is a precursor to the plant hormone indole-3-acetic acid.
Understanding auxin metabolism is crucial for plant growth regulation.

Purpose of the Study:

To confirm the presence of indoleacetaldehyde in cucumber cotyledons.
To investigate the metabolic fate of indoleacetaldehyde in cucumber seedlings.

Main Methods:

Thin layer chromatography (TLC) with three solvent systems.
Formation and hydrolysis of a bisulfite adduct.
Chemical reduction to indoleethanol and oxidation to indoleacetic acid.
Bioassays for quantification.
Exogenous application and tissue analysis.

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Main Results:

Indoleacetaldehyde was definitively identified in cucumber cotyledons.
A minimum content of 0.7 µg/kg fresh weight was determined in etiolated cotyledons.
Cucumber seedlings, both green and etiolated, reduced supplied indoleacetaldehyde to indoleethanol.

Conclusions:

Indoleacetaldehyde is naturally present in cucumber cotyledons.
Cucumber seedlings possess the metabolic capability to convert indoleacetaldehyde to indoleethanol.