Membrane Potential in Phaeoceros laevis: Effects of Anoxia, External Ions, Light, and Inhibitors

Gametophyte cells of Phaeoceros laevis (L.) Prosk. have vacuole electric potentials (PDs) of about -175 millivolts; the steady PD is not affected by light but small transient PDs result after changing from light to darkness or darkness to light. The PD is more negative than the Nernst potentials for any of the permeating ions. Changes in the concentration of any one of the external ions between 0.1 and 10 mm have only a very small effect on the PD. Increases in external pH cause the PD to depolarize by a few millivolts. Azide, 2,4-dinitrophenol, and NH(4)Cl each cause rapid and reversible depressions of the PD; the effects of these agents are similar in magnitude in the light and in the dark. Anoxia depolarizes the PD by about 30 millivolts in the light and by about 60 millivolts in the dark. Ouabain and 3-(3,4-dichlorophenyl)-1,1-dimethylurea have no effects on the PD. It is concluded that the membrane potential is controlled by an electrogenic efflux pump, possibly for H(+). It is also concluded that the source of energy for the pump is respiration and not photosynthesis.