Related Experiment Video
Updated: Aug 1, 2026

Measuring Fluxes of Mineral Nutrients and Toxicants in Plants with Radioactive Tracers
Published on: August 22, 2014
Metabolism of Ammonium Ion and Glutamate in Relation to Nitrogen Supply and Utilization during Grain Development in
1Department of Agricultural Biochemistry, School of Agriculture, University of Edinburgh, West Mains Road, Edinburgh EH9 3JG, Scotland.
Abstract:
Changes in the activity of a number of enzymes concerned with amino acid synthesis and metabolism were recorded for the endosperm, testa pericarp, and embryo of developing barley (Hordeum distichum L.) grains. Both glutamate-pyruvate transaminase and glutamate-oxaloacetate transaminase activities were present in all tissues and at all ages examined. Glutamate dehydrogenase activity was largely confined to endosperm while glutamine synthetase activity was mainly in the testa pericarp.Ammonium ion concentration was maximal in endosperm by 20 days after anthesis. Glutamate concentration varied in endosperm and was in the range of 3.5 to 8.5 mm between 20 and 45 days after anthesis. Significant levels of ammonium ion and glutamate were also present in the testa pericarp over the major part of the developmental period.
More Related Videos
10:29Calibrated Passive Sampling - Multi-plot Field Measurements of NH3 Emissions with a Combination of Dynamic Tube Method and Passive Samplers
Published on: March 21, 2016
12:47Workflow Based on the Combination of Isotopic Tracer Experiments to Investigate Microbial Metabolism of Multiple Nutrient Sources
Published on: January 22, 2018
Related Concept Videos
Urea Cycle
Overview of Metabolism
Plant Metabolism
Sunlight, the primary source of energy in plants, is first absorbed by the chlorophyll pigments present in their leaves. Plants then use this energy to carry out photosynthesis, where water is oxidized into oxygen and carbon dioxide...
Overview of Nitrogen Metabolism
The largest pool of nitrogen available in the terrestrial ecosystem is gaseous nitrogen (N2) from the air, but this nitrogen...
Preparation of Amines: Alkylation of Ammonia and Amines
Each alkylation step makes the nitrogen center more nucleophilic, which triggers successive alkylations until a quaternary ammonium salt is formed. Considering...
Inorganic Nitrogen Assimilation
Amino Acid Biosynthetic Pathways