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Updated: Aug 8, 2026

Plant Sample Preparation for Nucleoside/Nucleotide Content Measurement with An HPLC-MS/MS
Published on: February 24, 2021
Purine nucleotide metabolism of germinating soybean embryonic axes
1Post-Harvest Plant Physiology Laboratory, Agricultural Marketing Research Institute, Beltsville Agricultural Research Center, Science and Education Administration, SEA, United States Department of Agriculture, Beltsville, Maryland 20705.
Abstract:
Isolated soybean (Glycine max L. cv. Kent) embyronic axes metabolized [(14)C]glycine to ATP within the 1 hour of imbibition. Radioactivity was not detected in GTP until the 3rd hour. Throughout most of the first 24 hours of germination about 10 to 26 times as much label from [(14)C]glycine appears in ATP as GTP. About five times as much [(14)C]hypoxanthine and [(14)C]inosine was converted into GTP as into ATP in embryonic axes. Two independent pools of IMP appear to be used in purine nucleotide synthesis of soybean axes.
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