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Related Experiment Videos

Ethylene production by apple protoplasts.

J D Anderson1, M Lieberman, R N Stewart

  • 1Post Harvest Plant Physiology Laboratories and Ornamentals Laboratory, BARC-W, United States Department of Agriculture, Beltsville, Maryland 20705.

Plant Physiology
|May 1, 1979
PubMed
Summary
This summary is machine-generated.

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Apple protoplasts were isolated and their ethylene production was inhibited by osmotic shock, Triton X-100, and aminoethoxyvinyl glycine. Protease treatment did not significantly impact protoplasts or the ethylene system.

Area of Science:

  • Plant Physiology
  • Biochemistry
  • Cell Biology

Background:

  • Ethylene is a crucial plant hormone regulating various physiological processes.
  • Understanding ethylene biosynthesis and signaling in fruit tissues like apples is vital for agriculture.

Purpose of the Study:

  • To isolate functional protoplasts from ethylene-producing apple tissue.
  • To investigate the effects of specific treatments on ethylene production in these protoplasts.

Main Methods:

  • Isolation of protoplasts from apple fruit tissue.
  • Application of osmotic shock, Triton X-100, and aminoethoxyvinyl glycine to protoplasts.
  • Assessment of ethylene production and protoplast integrity.

Main Results:

Related Experiment Videos

  • Functional protoplasts were successfully isolated from apple tissue.
  • Ethylene production was significantly inhibited by osmotic shock, 0.01% Triton X-100, and aminoethoxyvinyl glycine.
  • Protease treatment showed minimal impact on both protoplast viability and the ethylene production system.

Conclusions:

  • Specific chemical and physical treatments can effectively modulate ethylene production in apple protoplasts.
  • Protoplast isolation methods are compatible with studying ethylene biosynthesis pathways.
  • These findings provide a foundation for further research into ethylene regulation in apples.