Related Experiment Video
Updated: Aug 8, 2026

An Induction System for Clustered Stomata by Sugar Solution Immersion Treatment in Arabidopsis thaliana Seedlings
Published on: February 15, 2019
Direct evidence for a sugar transport mechanism in isolated vacuoles
M Guy1, L Reinhold, D Michaeli
1Department of Botany, The Hebrew University of Jerusalem, Israel.
Abstract:
Sugar transport has been directly observed in isolated higher plant vacuoles for the first time. The latter were released from protoplasts isolated from the mesophyll of Pisum sativum L.Uptake of l-glucose by the vacuoles was very slight in comparison with that of the d-glucose analog 3-O-methyl glucose (MeG), indicating, first, that a highly selective sugar uptake mechanism is seated in the tonoplast; and, second, that the mechanism was functioning in the isolated vacuoles.MeG uptake was markedly sensitive to the pH of the medium, falling as the external pH rose. Addition of MgATP to buffered medium strongly promoted MeG uptake by vacuoles, but not by the protoplasts from which they were released. Treatment with the proton ionophore SF(6847) drastically reduced uptake by the vacuoles, but had a lesser effect on uptake by the protoplasts. The inhibitory effect of SF(6847) on uptake by the vacuoles was countered to a substantial degree by the addition of MgATP.The influence of pH, the stimulatory effect of ATP, and the ATP-reversible inhibition by SF(6847) all strengthen the conclusion that the observed sugar uptake reflected membrane function and was not due to a diffusional inward leak through damaged membranes.The results are discussed in the light of currently held concepts regarding the driving force for sugar transport.
More Related Videos
13:16Characterization of Membrane Transporters by Heterologous Expression in E. coli and Production of Membrane Vesicles
Published on: December 31, 2019
05:28Demonstration of Heterologous Complexes formed by Golgi-Resident Type III Membrane Proteins using Split Luciferase Complementation Assay
Published on: September 10, 2020
Related Concept Videos
Phloem and Sugar Transport
The Apoplast and Symplast
Short-distance Transport of Resources
Transcellular Transport of Solutes
Glucose Transporters
Facilitated diffusion-glucose transporters (GLUTs) are encoded by the solute-linked carrier (SLC) family 2, subfamily A gene family, or SLC2A. The 14 GLUT protein members are distributed into three classes:
Membrane Proteins