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Metabolic Pathway Confirmation and Discovery Through 13C-labeling of Proteinogenic Amino Acids
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Elongation Pathway for alpha-Linolenic Acid Synthesis in Spinach Leaves: A Reexamination
1Department of Biochemistry and Biophysics, University of California, Davis, California 95616.
Plant Physiology
|September 1, 1979
Abstract:
Leaf slices from spinach exhibited considerable variation in their incorporation of [(14)C]bicarbonate and [1-(14)C]acetate into fatty acids. Reductive ozonolysis studies indicated that all the (14)C-labeled fatty acids were synthesized de novo; no in vivo evidence was found for the previously proposed elongation of hexadecatrienoate to alpha-linolenate.
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