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Related Experiment Videos

Active cytokinins: photoaffinity labeling agents to detect binding.

R Mornet1, J B Theiler, N J Leonard

  • 1Roger Adams Laboratory, School of Chemical Sciences, University of Illinois, Urbana, Illinois 61801.

Plant Physiology
|October 1, 1979
PubMed
Summary

New azidopurine compounds show significant cytokinin activity, with 8-azido substitutions being over 10 times more potent than parent compounds. 8-Azidozeatin demonstrates exceptional activity, surpassing known cytokinins in plant growth promotion.

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Area of Science:

  • Plant Science
  • Biochemistry
  • Organic Chemistry

Background:

  • Cytokinins are essential plant hormones regulating cell division and growth.
  • Azidopurine derivatives offer a novel chemical scaffold for exploring cytokinin activity.

Purpose of the Study:

  • To synthesize and evaluate the cytokinin activity of four series of azidopurines.
  • To investigate the photolysis and reactivity of these novel compounds.

Main Methods:

  • Synthesis of 2- and 8-azido-N(6)-substituted adenines and zeatins.
  • Tobacco callus bioassay for cytokinin activity assessment.
  • Photolysis studies and mass spectrometry for product identification.
  • Competition assays with cytokinin-binding protein.

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Main Results:

  • 8-Azido substituted azidopurines exhibited 10-fold higher cytokinin activity than parent compounds.
  • 8-Azidozeatin showed potent activity at concentrations as low as 1.2 x 10(-5) micromolar.
  • Photolysis yielded products from nitrene intermediate reactions.
  • Azidopurine derivatives demonstrated potential for photoaffinity labeling of cytokinin-binding proteins.

Conclusions:

  • Azidopurine derivatives represent a promising class of potent cytokinin analogs.
  • The 8-azido substitution significantly enhances cytokinin activity.
  • These compounds can serve as valuable tools for studying cytokinin-binding proteins and their interactions.