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Updated: Aug 8, 2026

Analysis of Protein Import into Chloroplasts Isolated from Stressed Plants
Published on: November 1, 2016
Effects of pronase on isolated chloroplasts
L Fish1, V R Franceschi, C R Stocking
1Department of Botany, University of California, Davis, California 95616.
Abstract:
Subjecting isolated spinach chloroplasts to mild proteolysis (10-minute incubation at 20 C in 500 micrograms per milliliter pronase) caused chloroplast clumping but did not affect their integrity as measured by their ability to carry out light stimulated, glycerate-3-P-dependent O(2) evolution. Transmission electron microscopy revealed no detectable differences between the control and treated plastids. Mild proteolysis inactivated exogenously added pyruvate kinase and should be a useful technique in certain enzyme distribution studies.
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