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Related Experiment Videos

Light-induced Changes in Allophycocyanin.

I Ohad1, H J Schneider, S Gendel

  • 1The Biological Laboratories, Harvard University, Cambridge, Massachusetts 02138.

Plant Physiology
|January 1, 1980
PubMed
Summary

Allophycocyanin, a pigment in blue-green algae, exhibits light-induced absorbance changes, identifying it as the photoresponsive phycochrome. Its spectral properties and aggregation are pH-dependent and reversible.

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Area of Science:

  • Biochemistry
  • Phycology
  • Plant Physiology

Background:

  • Blue-green algae contain various pigments, including phycobiliproteins, which absorb light.
  • A previously unidentified
  • phycochrome
  • exhibiting light-induced absorbance changes was observed in algal extracts.

Purpose of the Study:

  • To identify the photoresponsive phycochrome in blue-green algae.
  • To characterize the spectral and aggregation properties of allophycocyanin.
  • To investigate the effect of pH on allophycocyanin's photoresponsiveness.

Main Methods:

  • Isoelectric focusing of algal extracts (Nostoc muscorum, Fremyella diplosiphon).
  • Selective immunoprecipitation of phycobiliproteins.
  • Spectrophotometric analysis of absorbance and fluorescence changes.
  • Sedimentation analysis (S(20,w)) to determine aggregation state.

Main Results:

  • Allophycocyanin was identified as the photoresponsive pigment responsible for light-induced absorbance changes.
  • Light-induced absorbance changes in allophycocyanin are reversible and dependent on illumination wavelength.
  • Allophycocyanin's spectral properties and aggregation state (monomer vs. aggregate) are pH-dependent and reversible, affecting its photoresponsiveness.

Conclusions:

  • Allophycocyanin is the previously unidentified phycochrome in blue-green algae.
  • The photoresponsiveness of allophycocyanin is linked to its spectral and aggregation properties, which are modulated by pH.
  • Further research is needed to determine if this phycochrome functions as an adaptochrome.

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