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Updated: Jun 25, 2026

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Chlorophyll determination in intact tissues using n,n-dimethylformamide.

R Moran1, D Porath

  • 1Department of Botany, The George S. Wise Faculty for Life Sciences, Tel Aviv University, Ramat Aviv, Israel.

Plant Physiology
|March 1, 1980
PubMed
Summary
This summary is machine-generated.

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A new method using N,N-dimethylformamide (DMF) efficiently extracts photosynthetic pigments from cucumber cotyledons. This technique saves time and prevents pigment loss, especially at low concentrations.

Area of Science:

  • Plant Physiology
  • Biochemistry

Background:

  • Photosynthetic pigments are crucial for plant energy production.
  • Efficient extraction methods are vital for accurate pigment analysis.
  • Traditional methods can be time-consuming and lead to pigment degradation.

Purpose of the Study:

  • To develop a novel and efficient method for extracting photosynthetic pigments.
  • To evaluate the effectiveness of N,N-dimethylformamide (DMF) as an extraction solvent.
  • To determine the specific absorption coefficient of chlorophyll a in DMF.

Main Methods:

  • Direct immersion of intact etiolated cucumber cotyledons into N,N-dimethylformamide (DMF).
  • Spectrophotometric analysis to quantify extracted pigments.
  • Determination of the specific absorption coefficient for chlorophyll a in DMF.

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Last Updated: Jun 25, 2026

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Main Results:

  • N,N-dimethylformamide (DMF) proved to be a highly efficient solvent for pigment extraction.
  • The direct immersion method saved time and tools compared to conventional techniques.
  • This method minimized pigment loss, particularly when pigment concentrations were low.
  • The specific absorption coefficient of chlorophyll a in DMF was successfully determined.

Conclusions:

  • Direct immersion in DMF is a superior method for extracting photosynthetic pigments from cucumber cotyledons.
  • This technique offers significant advantages in terms of efficiency, time-saving, and pigment preservation.
  • The findings provide a valuable new protocol for pigment analysis in plant science research.