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Updated: Aug 8, 2026

Flash-and-Freeze: A Novel Technique to Capture Membrane Dynamics with Electron Microscopy
10:01

Flash-and-Freeze: A Novel Technique to Capture Membrane Dynamics with Electron Microscopy

Published on: May 1, 2017

In vivo detection of membrane injury at freezing temperatures

D G Stout1, W Majak, M Reaney

  • 1Range Research Station, Agriculture Canada, Kamloops, British Columbia V2B 8A9 Canada.

Plant Physiology
|July 1, 1980
PubMed

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Cryo-electron Microscopy01:28

Cryo-electron Microscopy

Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...