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Capillary Electrophoresis-based Hydrogen/Deuterium Exchange for Conformational Characterization of Proteins with Top-down Mass Spectrometry
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Microheterogeneity of globulin-1 storage protein from French bean with isoelectrofocusing

J W Brown1, F A Bliss, T C Hall

  • 1Department of Horticulture, University of Wisconsin, Madison, Wisconsin 53706.

Plant Physiology
|November 1, 1980
PubMed

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Two-dimensional gel electrophoresis is a high-resolution protein separation method first introduced by O' Farrell and Klose in 1975. This method involves protein separation by two dimensions, mass and charge, making it more accurate than one-dimensional gel electrophoresis.
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