Related Experiment Video
Updated: Aug 8, 2026

AirID-Based Proximity Labeling for Protein-Protein Interaction in Plants
Published on: September 16, 2022
Ethylene Production by Chilled Cucumbers (Cucumis sativus L.)
1Horticultural Crops Quality and Postharvest Physiology Laboratories, Agricultural Research, Science and Education Administration, United States Department of Agriculture, Beltsville, Maryland 20705.
Abstract:
Chilling at 2.5 C accelerated the synthesis of 1-aminocyclopropane-1-carboxylic acid (ACC) and C(2)H(4) production in cucumber fruit. Skin tissue contained higher levels of ACC and was more sensitive to chilling than was cortex tissue. Accumulation of ACC in chilled tissue was detected after 1 day of chilling and remained elevated even after C(2)H(4) production started to decline. These data suggest that ACC synthesis is readily stimulated by chilling, whereas the system that converts ACC to C(2)H(4) is vulnerable to chilling injury. Chilling-induced C(2)H(4) production was inhibited by amino-ethoxyvinylglycine, sodium benzoate, propyl gallate, 2,4-dinitrophenol, carbonyl cyanide m-chlorophenylhydrazone, and cycloheximide. The utilization of methionine for ACC formation and chilling-induced C(2)H(4) biosynthesis was established using l-[3,4-(14)C]methionine. Chilled tissue had a higher capacity to convert l-[3,4-(14)C]methionine to ACC and C(2)H(4) than did nonchilled tissue.
Related Concept Videos
Microbes in the Production of Fermented Foods
Production of Organic Acids
Production of Antibiotics
Production of Pharmaceuticals

