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Electrophoretic comparison of polypeptides from enriched plasma membrane fractions from developing soybean roots
1Department of Agronomy and Range Science, University of California, Davis, California 95616.
Plant Physiology
|December 1, 1980
Summary
Soybean root plasma membranes were analyzed using two-dimensional gel electrophoresis. This method identified unique protein profiles for different soybean tissues, aiding in membrane characterization.
Area of Science:
- Plant Biology
- Molecular Biology
- Biochemistry
Background:
- Understanding the molecular composition of plant cell membranes is crucial for deciphering cellular functions.
- The plasma membrane plays a vital role in nutrient transport, signaling, and structural integrity in plant roots.
Purpose of the Study:
- To characterize the polypeptide complement of soybean (Glycine max [L.] Merr. cult. wells) root plasma membranes.
- To compare the protein profiles of plasma membranes with other cellular membranes.
- To investigate differences in plasma membrane protein complements between meristematic and mature root tissues.
Main Methods:
- Two-dimensional gel electrophoresis was employed to separate and visualize membrane polypeptides.
- Solubilization of polypeptides using sodium dodecyl sulfate and inclusion of butylated hydroxytoluene improved resolution.
- Comparative electrophoresis was performed on mitochondrial and endomembrane fractions.
Main Results:
- A characteristic electrophoretogram was obtained for the soybean root plasma membrane.
- Each membrane preparation (plasma membrane, mitochondrial, endomembranes) exhibited a distinct polypeptide profile.
- Qualitative and quantitative differences were observed in the protein complements of plasma membranes from meristematic versus mature root tissues.
Conclusions:
- Two-dimensional gel electrophoresis is an effective method for identifying and differentiating plant membrane types based on their protein composition.
- The protein complement of soybean root plasma membranes varies between different developmental stages of root tissue.
- This study provides a foundational proteomic dataset for soybean root plasma membranes.
Related Concept Videos
01:22Two-dimensional Gel Electrophoresis
Two-dimensional gel electrophoresis is a high-resolution protein separation method first introduced by O' Farrell and Klose in 1975. This method involves protein separation by two dimensions, mass and charge, making it more accurate than one-dimensional gel electrophoresis.
The first dimension separation uses the isoelectric focusing or IEF technique performed on immobilized pH gradient (IPG) strips that separate proteins according to their isoelectric points.
Biological samples, such as cells...
The first dimension separation uses the isoelectric focusing or IEF technique performed on immobilized pH gradient (IPG) strips that separate proteins according to their isoelectric points.
Biological samples, such as cells...
01:27SDS-PAGE
Gel electrophoresis is a method that separates biological macromolecules like nucleic acids or proteins by forcing them to pass through a gel matrix under an electric field.
A variation of gel electrophoresis, termed polyacrylamide gel electrophoresis (PAGE), is commonly used for separating proteins according to their molecular size by passing them through a polyacrylamide gel. Because of the varying charges associated with amino acid side chains, PAGE can be used to separate intact proteins...
A variation of gel electrophoresis, termed polyacrylamide gel electrophoresis (PAGE), is commonly used for separating proteins according to their molecular size by passing them through a polyacrylamide gel. Because of the varying charges associated with amino acid side chains, PAGE can be used to separate intact proteins...
01:15Western Blotting
Western blotting is an analytical technique for protein identification. It has various applications in immunology and medicine, including detecting diseases like bovine spongiform encephalopathy, mad cow disease, and human and feline immunodeficiency virus from biological samples.
The technique begins with separating proteins from the sample using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), followed by protein transfer, immunoblotting, and finally, protein detection.
The technique begins with separating proteins from the sample using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), followed by protein transfer, immunoblotting, and finally, protein detection.