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Updated: Aug 8, 2026

A Rapid and Specific Microplate Assay for the Determination of Intra- and Extracellular Ascorbate in Cultured Cells
Published on: April 11, 2014
Vacuolar Deposition of Ascorbate-derived Oxalic Acid in Barley
1Department of Biology, Brookhaven National Laboratory, Upton, New York 11973.
Abstract:
l-[1-(14)C]Ascorbic acid was supplied to detached barley seedlings to determine the subcellular location of oxalic acid, one of its metabolic products. Intact vacuoles isolated from protoplasts of labeled leaves contained [(14)C]oxalic acid which accounted for about 70% of the intraprotoplast soluble oxalic acid. Tracer-labeled oxalate accounted for 36 and 72% of the (14)C associated with leaf vacuoles of seedlings labeled for 22 and 96 hours, respectively.
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