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Related Experiment Videos

Electrical potentials in stomatal complexes.

R A Saftner1, K Raschke

  • 1MSU-DOE Plant Research Laboratory, Michigan State University, East Lansing, Michigan 48824.

Plant Physiology
|June 1, 1981
PubMed
Summary
This summary is machine-generated.

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Guard cell electrical potential differences were measured across plant species. Results show these potentials correlate with stomatal opening and ion concentrations, impacting plant responses.

Area of Science:

  • Plant Physiology
  • Cellular Electrophysiology

Background:

  • Guard cells regulate stomatal aperture, controlling gas exchange in plants.
  • Understanding guard cell electrophysiology is crucial for plant signaling research.

Purpose of the Study:

  • To investigate the electrical potential differences within guard cells of various plant species.
  • To correlate these potentials with stomatal aperture and ionic environment.

Main Methods:

  • Micropipette electrodes were used to measure potential differences in guard cells.
  • Experiments were conducted on Commelina communis and other species in controlled bathing media.

Main Results:

  • Guard cell walls exhibit Donnan potentials (-60 to -70 mV) due to fixed charges.

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  • Vacuolar potentials range from -40 to -50 mV (open stomata) and -30 mV (closed stomata).
  • Potentials correlate linearly with stomatal aperture and respond to ionic strength; Li+ and Ca2+ show specific effects.

    • Conclusions:

    • Electrical potentials in guard cells are dynamic and linked to stomatal function.
    • Ion concentrations, particularly Ca2+, significantly influence guard cell electrical behavior and stomatal closure.
    • Electrical potential gradients across stomatal complexes change with stomatal state.