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Updated: Aug 8, 2026

Isolation of Physiologically Active Thylakoids and Their Use in Energy-Dependent Protein Transport Assays
Published on: September 28, 2018
Effect of temperature on proton efflux from isolated chloroplast thylakoids
1Biology Department, Georgia State University, University Plaza, Atlanta, Georgia 30303.
Abstract:
Temperature-induced changes in the decay of the light-induced proton gradient of chloroplast thylakoids isolated from chilling-resistant and chilling-sensitive plants have been examined. In the presence of N-methylphenazonium methosulfate, the thylakoids isolated from chilling-resistant barley (cv. Kanby) and pea (cv. Alaska) and chilling-sensitive mung bean (cv. Berken) plants showed temperature-induced changes at approximately 8.6, 13.3, and 14.0 C, respectively. Barley thylakoids assayed in the presence of sodium thiocyanate also showed a change at 8.6 C, whereas with no addition or upon the inclusion of both N-methylphenazonium methosulfate and sodium thiocyanate the change occurred at approximately 11.5 C.Temperature-induced changes in electron transport activities occurred at temperatures approximating those for proton efflux for each of the three plants. These results indicate that temperature has a general effect on thylakoid membranes and that chloroplast thylakoids from chilling-resistant and chilling-sensitive plants have their proton permeability properties affected similarly by temperature.
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