Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Formate oxidation and oxygen reduction by leaf mitochondria.

D J Oliver1

  • 1Department of Bacteriology and Biochemistry, University of Idaho, Moscow, Idaho 83843.

Plant Physiology
|September 1, 1981
PubMed
Summary

Plant leaf mitochondria contain NAD-linked formate dehydrogenase, producing NADH for ATP synthesis. Activity varies by species, with spinach showing significant formate oxidation, raising questions about its metabolic role.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Patient and caregiver involvement in the formulation of guideline questions: findings from the European Academy of Neurology guideline on palliative care of people with severe multiple sclerosis.

European journal of neurology·2018
Same author

European Academy of Neurology/European Association for Palliative Care Taskforce on Neurology Consensus recommendations on palliative care for patients with chronic and progressive neurological disease - acceptability for Belgian neurologists.

European journal of neurology·2017
Same author

A consensus review on the development of palliative care for patients with chronic and progressive neurological disease.

European journal of neurology·2015
Same author

Specialist palliative care improves the quality of life in advanced neurodegenerative disorders: NE-PAL, a pilot randomised controlled study.

BMJ supportive & palliative care·2015
Same author

One-to-one spatially matched experiment and atomistic simulations of nanometre-scale indentation.

Nanotechnology·2013
Same author

E. coli propionyl-CoA synthetase is regulated in vitro by an intramolecular disulfide bond.

Prikladnaia biokhimiia i mikrobiologiia·2012

Area of Science:

  • Plant biochemistry
  • Mitochondrial respiration
  • Enzyme activity

Background:

  • Mitochondria are key organelles for cellular respiration in plants.
  • NAD-linked enzymes play crucial roles in metabolic pathways.
  • Formate dehydrogenase activity in plant mitochondria is not well-characterized.

Purpose of the Study:

  • To investigate the presence and activity of NAD-linked formate dehydrogenase in plant leaf mitochondria.
  • To determine the contribution of this enzyme to cellular energy production.
  • To explore species-specific variations and potential physiological roles.

Main Methods:

  • Isolation of mitochondria from leaves of various plant species.
  • Assay of NAD-linked formate dehydrogenase activity.
  • Measurement of NADH oxidation coupled to electron transport.
  • Determination of oxygen uptake rates.

Main Results:

  • NAD-linked formate dehydrogenase was detected in mitochondria from several plant species.
  • Enzyme activity and capacity for formate-dependent oxygen uptake varied significantly among species.
  • Soybean leaf mitochondria showed no detectable activity.
  • Spinach leaf mitochondria exhibited formate oxidation rates approximately half those of malate oxidation.
  • Only mitochondria from green spinach tissues oxidized formate.

Conclusions:

  • Plant leaf mitochondria possess NAD-linked formate dehydrogenase, linking formate oxidation to ATP synthesis.
  • Significant species- and tissue-specific variations in activity suggest specialized roles.
  • The physiological significance and source of formate for this pathway require further investigation.

Related Experiment Videos