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Cytokinins from the Moss Physcomitrella patens.

T L Wang1, R Horgan, D Cove

  • 1Department of Genetics, University of Leeds, Leeds, LS2 9JT United Kingdom.

Plant Physiology
|September 1, 1981
PubMed
Summary

Mutant mosses (Physcomitrella patens) overproduce cytokinin, a plant hormone, in liquid cultures. This excess hormone explains their unusual growth patterns, mimicking plants treated with external cytokinin.

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Area of Science:

  • Plant biology
  • Molecular genetics
  • Biochemistry

Background:

  • Gametophore-over-producing mutants of Physcomitrella patens exhibit an unusual phenotype.
  • This phenotype resembles that of mosses treated with exogenous (external) cytokinin.
  • Previous studies identified N(6)-(Delta(2)-isopentenyl)adenine in some of these mutants.

Purpose of the Study:

  • To identify the specific cytokinins produced by gametophore-over-producing Physcomitrella patens mutants.
  • To confirm the role of endogenous cytokinin in the observed mutant phenotype.

Main Methods:

  • Culturing Physcomitrella patens mutants in liquid media.
  • High-performance liquid chromatography (HPLC) for cytokinin separation.
  • Mass spectrometry (MS), including chemical ionization MS of permethyl derivatives, for cytokinin identification.

Main Results:

  • High levels of cytokinin were detected in the culture medium of the mutants.
  • N(6)-(Delta(2)-isopentenyl)adenine was identified in additional mutant representatives.
  • Zeatin was identified as a second major cytokinin based on chromatographic and mass spectral data.

Conclusions:

  • Gametophore-over-producing Physcomitrella patens mutants synthesize and export significant amounts of cytokinin.
  • The identified cytokinins, N(6)-(Delta(2)-isopentenyl)adenine and zeatin, likely account for the mutants' characteristic phenotype.
  • This study confirms the endogenous production of specific cytokinins driving developmental changes in moss mutants.

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