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Related Experiment Videos

l-Ascorbic Acid Biosynthesis in Ochromonas danica.

J P Helsper1, L Kagan, C L Hilby

  • 1Institute of Biological Chemistry and Program in Biochemistry and Biophysics, Washington State University, Pullman, Washington 99164.

Plant Physiology
|February 1, 1982
PubMed
Summary
This summary is machine-generated.

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Ochromonas danica converts glucose to l-ascorbic acid using a unique pathway. Unlike animals, it utilizes d-galacturonate and l-galactono-1,4-lactone for enhanced vitamin C production.

Area of Science:

  • Biochemistry
  • Microbiology
  • Algal Metabolism

Background:

  • Ochromonas danica, a freshwater chrysomonad, is known to synthesize l-ascorbic acid (vitamin C).
  • The metabolic pathway involves inversion of the sugar carbon chain, similar to animal synthesis.

Purpose of the Study:

  • To investigate the specific regulatory mechanisms and precursors involved in l-ascorbic acid synthesis in Ochromonas danica.
  • To compare and contrast the algal pathway with known animal pathways for vitamin C production.

Main Methods:

  • Metabolic analysis of Ochromonas danica.
  • Tracing the incorporation of radiolabeled d-glucose (d-[1-(14)C]glucose).
  • Assessing the effects of d-galacturonate, l-galactono-1,4-lactone, d-glucuronate, and l-gulono-1,4-lactone on ascorbic acid production.

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Main Results:

  • Ochromonas danica converts d-glucose to l-ascorbic acid via a carbon-inverting pathway.
  • d-galacturonate and l-galactono-1,4-lactone significantly enhance l-ascorbic acid production.
  • These specific galacturonate derivatives also repress the incorporation of (14)C from d-[1-(14)C]glucose into ascorbic acid.
  • d-glucuronate and l-gulono-1,4-lactone do not show the same enhancing or repressing effects.

Conclusions:

  • Ochromonas danica possesses a distinct regulatory mechanism for l-ascorbic acid synthesis compared to animals.
  • The pathway is modulated by specific galacturonate derivatives, suggesting unique enzymatic or transport processes.