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Related Experiment Videos

Citrate transport in corn mitochondria.

P R Birnberg1, D L Jayroe, J B Hanson

  • 1Department of Botany, University of Illinois, Urbana, Illinois 61801.

Plant Physiology
|August 1, 1982
PubMed
Summary

Corn mitochondria rapidly accumulate citrate via H+/citrate co-transport, a mechanism distinct from dicarboxylate exchange. This process is crucial for understanding plant energy metabolism.

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Area of Science:

  • Plant Physiology
  • Mitochondrial Transport
  • Biochemistry

Background:

  • Citrate is a key metabolic intermediate in plants, particularly within mitochondria.
  • Understanding citrate transport mechanisms is vital for elucidating plant energy metabolism and cellular regulation.

Purpose of the Study:

  • To investigate the mechanisms of citrate uptake in corn (Zea mays L. B73 x Mol9) mitochondria.
  • To differentiate between various potential transport pathways, including passive influx, gradient-driven uptake, respiration-driven transport, and carrier-mediated exchange.

Main Methods:

  • Osmotic swelling assays to assess mitochondrial volume changes.
  • Radiolabeled [(14)C]citrate accumulation studies.
  • Experiments utilizing ionophores, uncouplers, and inhibitors of specific mitochondrial transporters.
  • Investigating the influence of pH, phosphate, malate, and ammonium gradients on citrate uptake.

Main Results:

  • Citrate uptake was observed independent of phosphate or malate, though these could be promotive.
  • Inhibiting phosphate and dicarboxylate carriers did not abolish citrate uptake.
  • Citrate/malate exchange occurred but was too slow to explain the observed uptake rates; malate depletion reduced uptake by only 38%.
  • Respiration-driven citrate accumulation was sensitive to uncouplers.
  • Passive swelling studies indicated H+/citrate co-transport as the primary uptake route, with a pH optimum around 4.5.

Conclusions:

  • Corn mitochondria rapidly accumulate citrate through a mechanism other than dicarboxylate exchange.
  • The predominant pathway for citrate uptake is H+/citrate co-transport.
  • The physiological role of this rapid H+/citrate co-transport in vivo remains to be determined.

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