Effect of pod removal on leaf senescence in soybeans
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Updated: Aug 8, 2026

A Simple Method for Isolation of Soybean Protoplasts and Application to Transient Gene Expression Analyses
Published on: January 25, 2018
1Central Research and Development Department, Experimental Station, E. I. du Pont de Nemours and Company, Wilmington, Delaware 19898.
Abstract:
Depodding soybean (Glycine max [L] Merr. cv Wye) plants results in an apparent inhibition of senescence as indicated by leaf chlorophyll and soluble protein retention. However, leaf photosynthesis and ribulose bisphosphate carboxylase (Rubisco) levels begin to decline earlier in depodded than in control, podded plants. The initial decline in photosynthesis is correlated with a decrease in leaf transpiration, while the latter decline is associated with the loss of Rubisco. Total soluble protein remains high in depodded plants because several polypeptides, three in particular, increase in amounts sufficient to offset the loss of Rubisco. Thus, depodding appears to change the function of the leaf rather than simply delaying or preventing the decline in leaf function. Changes in specific leaf weight and starch content following depodding suggest that the leaf may be changing to a storage organ.
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