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Updated: Aug 8, 2026

Pull-down of Calmodulin-binding Proteins
Published on: January 23, 2012
Calmodulin-binding drugs affect responses to cytokinin, auxin, and gibberellic Acid
D C Elliott1, S M Batchelor, R A Cassar
1School of Biological Sciences, Flinders University of South Australia, Bedford Park, South Australia 5042.
Abstract:
Trifluoperazine, a phenothiazine tranquilizer, and tetracaine, a local anesthetic, have been found to inhibit a variety of plant hormone responses at concentrations compatible with their known inhibition of Ca(2+)-calmod-ulin-dependent enzyme activities. Among these responses are cytokinin-dependent betacyanin synthesis and increase in fresh weight in Amaranthus tricolor cotyledons, auxin-dependent increase in length of wheat coleoptile segments and gibberellic acid-dependent induction of alpha-amylase synthesis in barley aleurone layers. The reversibility of some of these inhibitory effects has been demonstrated, indicating that, up to a point, a generalized membrane destruction can be ruled out. The evidence, taken in conjunction with numerous examples from the literature showing calcium involvement in the action of all of the plant hormones, support a unifying theory of hormone action.
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