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Updated: Aug 8, 2026

Imaging Spatial Reorganization of a MAPK Signaling Pathway Using the Tobacco Transient Expression System
Published on: March 20, 2016
Shikimate Pathway Activity during Shoot Initiation in Tobacco Callus Cultures
L D Beaudoin-Eagan1, T A Thorpe
1Plant Physiology Research Group, Department of Biology, University of Calgary, Calgary, Alberta T2N 1N4 Canada.
Abstract:
The activity of the shikimic acid pathway during shoot initiation in tobacco (Nicotiana tabacum L. Wisconsin 38) callus was examined. Enhancement of the activities of 3-deoxy-d-arabino-heptulosonic acid 7-phosphate synthase, shikimate kinase, chorismate mutase, and anthranilate synthase was observed during culture of tobacco callus under shootforming conditions in comparison to tissue cultured under non-organforming conditions. Confirmation of these findings was obtained by examining the incorporation of d-[(14)C]glucose into quinic and shikimic acids and of [(14)C]shikimic acid into tyrosine, phenylalanine, and tryptophan.
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