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Published on: June 25, 2018
Characterization of Radish (Raphanus sativus) Storage Proteins
M Laroche1, L Aspart, M Delseny
1Laboratoire de Physiologie Végétale-E.R.A. No. 226 du CNRS, Université de Perpignan, 66025 Perpignan-Cedex, France.
Plant Physiology
|March 1, 1984
Summary
Radish seeds contain two main storage protein fractions, 12 Svedberg and 1.7 Svedberg. These proteins differ in polypeptide composition, subunit structure, and amino acid profiles, offering insights into cruciferous seed storage proteins.
Area of Science:
- Plant biochemistry
- Seed storage proteins
- Proteomics
Background:
- Cruciferous oil seeds, including radish (Raphanus sativus), store proteins in aggregate forms.
- Two major protein fractions, 12 Svedberg and 1.7 Svedberg, are typically found in these seeds.
Purpose of the Study:
- To characterize the protein composition and structure of radish seed storage protein aggregates.
- To compare radish storage proteins with those found in rapeseed.
Main Methods:
- Gel filtration chromatography to separate protein fractions.
- Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) for polypeptide analysis.
- Amino acid composition analysis.
- Bidimensional gel electrophoresis systems.
Main Results:
- Radish 12 Svedberg particles consist of nine major polypeptides (30-33 kDa) with charge heterogeneity and six subunits (approx. 55 kDa), each a disulfide-linked dimer.
- Radish 1.7 Svedberg particles are simpler, containing 10-12 kDa polypeptides and ~7 kDa peptides, with a basic amino acid profile rich in glutamic acid and proline.
- Rapeseed storage protein organization shows similarities but is more complex than radish.
Conclusions:
- Radish seed storage proteins exhibit distinct structural and compositional characteristics between the 12 Svedberg and 1.7 Svedberg fractions.
- The findings provide a detailed molecular understanding of radish seed storage proteins, aiding comparative studies within the Brassicaceae family.
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