Related Experiment Video
Updated: Aug 8, 2026

Discrimintion and Mapping of the Primary and Processed Transcripts in Maize Mitochondrion Using a Circular RT-PCR-based Strategy
Published on: July 29, 2019
Protein synthesis patterns : relevance of old and new messenger RNA in germinating maize embryos
1Departamento de Bioquímica Vegetal, Facultad de Química, UNAM, México, D.F., Mexico.
Abstract:
The proteins synthesized during the first hours of seed imbibition were studied in axes and scutellum of maize embryos separately. Increase in fresh weight was followed in the embryonic axes through the germination period. Pulse labeling experiments with (14)C-amino acids were carried out at two stages of development: 0 to 6 and 18 to 24 hours in the presence and absence of alpha-amanitin. The proteins were analyzed by two-dimensional gel electrophoresis and fluorography. Results showed a major pattern of proteins common to both tissues, axes and scutellum (;house keeping' proteins), besides the specific proteins synthesized by each tissue. In the axes, the changes in proteins observed between the periods of 0 to 6 and 18 to 24 hours of development seem to be due both to newly synthesized mRNA as well as to delayed translation of stored mRNA species.
Related Concept Videos
pre-mRNA Processing
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a “cap” to the 5’ end of the growing transcript. In this process, a 5’ phosphate is replaced by modified guanosine that has a methyl group attached to it (7-Methyl guanosine). This 5’ cap helps the...
Leaky Scanning
Regulation of Expression Occurs at Multiple Steps
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
Types of RNA
RNA Performs Diverse...
Types of RNA
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
Pre-mRNA Processing: Modification of pre-mRNA Ends
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a cap to the 5' end of the growing transcript. In this process, a 5' phosphate is replaced by modified guanosine that has a methyl group attached (7-methyl guanosine). This 5' cap helps the cell...
