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Updated: Jun 24, 2026

Isolation of Protoplasts from Tissues of 14-day-old Seedlings of Arabidopsis thaliana
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Suberin production by isolated tomato fruit protoplasts.

G S Rao1, J H Willison, W M Ratnayake

  • 1Biology Department, Dalhousie University, Halifax, Nova Scotia B3H 4J1 Canada.

Plant Physiology
|July 1, 1984
PubMed
Summary
This summary is machine-generated.

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Researchers identified suberin in tomato fruit walls. Analysis of depolymerized extracts revealed specific fatty acids, alcohols, and esters, confirming suberin's presence and composition.

Area of Science:

  • Plant Biology
  • Biochemistry
  • Agricultural Science

Background:

  • Plant cell walls play crucial roles in fruit development and protection.
  • Suberin is a complex biopolymer found in plant tissues, contributing to barrier functions.

Purpose of the Study:

  • To isolate and characterize the multilamellar wall component from tomato fruit locule tissue.
  • To identify the chemical constituents of this wall material to confirm the presence of suberin.

Main Methods:

  • Protoplasts were isolated from tomato locule tissue.
  • The secreted multilamellar wall was purified and depolymerized using BF(3)-methanol.
  • Extracts were analyzed using thin layer chromatography (TLC) and gas chromatography (GC).
  • Quantification was performed using an Iatroscan TLC-flame ionization detection system.

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Last Updated: Jun 24, 2026

Isolation of Protoplasts from Tissues of 14-day-old Seedlings of Arabidopsis thaliana
11:16

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Published on: August 17, 2009

Isolation and Biophysical Study of Fruit Cuticles
15:53

Isolation and Biophysical Study of Fruit Cuticles

Published on: March 30, 2012

Protein-protein Interactions Visualized by Bimolecular Fluorescence Complementation in Tobacco Protoplasts and Leaves
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Protein-protein Interactions Visualized by Bimolecular Fluorescence Complementation in Tobacco Protoplasts and Leaves

Published on: March 9, 2014

Main Results:

  • TLC analysis revealed the presence of fatty acid methyl esters, fatty alcohols, dicarboxylic acid dimethyl esters, and omega-hydroxy acid methyl esters.
  • GC analysis identified and quantified the different chain lengths of these components.
  • The chemical profile strongly indicated the presence of suberin.

Conclusions:

  • The multilamellar wall secreted by tomato fruit protoplasts is composed of suberin.
  • This study provides detailed chemical characterization of suberin from tomato fruit tissue.