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Published on: August 17, 2009
Suberin production by isolated tomato fruit protoplasts
G S Rao1, J H Willison, W M Ratnayake
1Biology Department, Dalhousie University, Halifax, Nova Scotia B3H 4J1 Canada.
Abstract:
The multilamellar wall secreted by protoplasts isolated from locule tissue of tomato (Lycopersicon esculentum L.) fruit was purified, and an extract was obtained after depolymerization with BF(3)-methanol. Analysis of this extract using thin layer chromatography demonstrated the presence of fatty acid methyl esters, fatty alcohols, dicarboxylic acid dimethyl esters, and omega-hydroxy acid methyl esters. These components were quantified using an Iatroscan thin layer chromatography-flame ionization detection system. The different chain lengths in each group were identified and quantified using gas chromatography. The results clearly indicated the presence of suberin.
