Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Extensor and flexor protoplasts from samanea pulvini : I. Isolation and initial characterization.

H L Gorton1, R L Satter

  • 1Biological Sciences Group U-42, University of Connecticut, Storrs, Connecticut 06268.

Plant Physiology
|November 1, 1984
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Isolation of soluble metabolites of the phosphatidylinositol cycle from Samanea saman.

Plant cell reports·2013
Same author

Effects of white, blue, red light and darkness on pH of the apoplast in the Samanea pulvinus.

Planta·2013
Same author

Light-promoted changes in apoplastic K(+) activity in the Samanea saman pulvinus, monitored with liquid membrane microelectrodes.

Planta·2013
Same author

The light environment and cellular optics of the snow alga Chlamydomonas nivalis (Bauer) Wille.

Photochemistry and photobiology·2001
Same author

Interaction of the Depolarization-Activated K Channel of Samanea saman with Inorganic Ions: A Patch-Clamp Study.

Plant physiology·1990
Same author

Circadian Stomatal Rhythms in Epidermal Peels from Vicia faba.

Plant physiology·1989

Protoplast isolation from Samanea saman pulvini reveals metabolic activity and functional proton transport. Isolated cells adapt to conditions mimicking a closed pulvinus, despite initial structural changes.

Area of Science:

  • Plant Physiology
  • Cell Biology

Background:

  • Nyctinastic movement in plants like Samanea saman involves pulvini, specialized structures regulating leaf orientation.
  • Pulvini contain distinct cell types (extensor and flexor) with differing physiological characteristics in vivo.

Purpose of the Study:

  • To isolate and characterize protoplasts from extensor and flexor regions of Samanea saman pulvini.
  • To assess the metabolic activity and proton transport functionality of isolated pulvini protoplasts.

Main Methods:

  • Protoplast isolation from extensor and flexor tissues of open pulvini.
  • Observation of protoplast vacuolar structure and osmotic pressure.
  • Measurement of proton extrusion in response to fusicoccin.

Main Results:

Related Experiment Videos

  • Both extensor and flexor protoplasts were successfully isolated using 0.5 M sorbitol.
  • Isolated protoplasts exhibited changes in vacuolar structure and osmotic pressure compared to in vivo cells.
  • Protoplasts demonstrated proton extrusion upon fusicoccin treatment, indicating metabolic activity.

Conclusions:

  • Isolated Samanea saman pulvini protoplasts retain metabolic activity and functional proton transport mechanisms.
  • The observed changes in protoplasts suggest they adopt characteristics of cells found in a closed pulvinus environment.
  • Pulvini protoplast isolation is a viable method for studying cellular mechanisms in nyctinastic plants.