Related Experiment Videos
Quantifying Apoplastic Flux through Red Pine Root Systems Using Trisodium, 3-hydroxy-5,8,10-pyrenetrisulfonate.
P J Hanson1, E I Sucoff, A H Markhart
1Plant Physiology Program, University of Minnesota, St. Paul, Minnesota 55108.
Plant Physiology
|January 1, 1985
Summary
Anaerobic conditions significantly increase apoplastic water flow in red pine roots, allowing up to 50% of total flux without crossing membranes. This effect is reversible, indicating a dynamic pathway shift in Pinus resinosa Ait. root systems.
Area of Science:
- Plant Physiology
- Water Transport Mechanisms
- Root Biology
Background:
- The apoplast pathway is a route for water movement in plant roots.
- Understanding water flux dynamics is crucial for plant survival and function.
- Red pine (Pinus resinosa Ait.) is an important coniferous species.
Purpose of the Study:
- To quantify apoplastic flux in red pine root systems.
- To investigate the impact of anaerobic conditions on water transport pathways.
- To determine if pressure gradients influence apoplastic contribution.
Main Methods:
- Utilized the fluorescent compound trisodium, 3-hydroxy-5,8,10-pyrenetrisulfonate (PTS) as a tracer.
- Measured water flux under pressure in detopped roots and transpiration in intact seedlings.
- Calculated apoplastic flux based on PTS concentration ratios in xylem exudate and bathing medium.
Main Results:
- Under aerobic conditions, apoplastic flux was less than 1% of total flux.
- Under anaerobic conditions, apoplastic flux increased to up to 50% of total flux.
- The shift in water pathway under anaerobic conditions was reversible and observed in both experimental setups.
Conclusions:
- Anaerobic conditions dramatically alter water movement into the root xylem of red pine.
- The root's water transport pathway is dynamic and responsive to oxygen availability.
- Apoplastic water flow in red pine roots can be significantly influenced by environmental conditions and pressure gradients.