Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Controls on na influx in corn roots.

B Jacoby1, J B Hanson

  • 1Department of Plant Biology, University of Illinois, Urbana, Illinois 61801.

Plant Physiology
|April 1, 1985
PubMed
Summary

Sodium (Na+) influx in corn roots is influenced by cell membrane potential and ion presence. Calcium (Ca2+) consistently inhibits Na+ influx, while potassium (K+) affects energy-linked transport.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The effect of 2,4-dichlorophenoxyacetic acid upon the metabolism and composition of soybean hypocotyl mitochondria.

Planta·2014
Same author

Oligomycin effect on ion absorption by excised barley roots and on their ATP content.

Planta·2014
Same author

Perfusion of onion root xylem vessels: a method and some evidence of control of the pH of the xylem sap.

Planta·2013
Same author

External fixation of distal radial fractures: 3 or 5 weeks of external fixation.

International orthopaedics·2000
Same author

Classification of otitis media and surgical principles.

Otolaryngologic clinics of North America·1999
Same author

Clinical picture. Lightning burns.

Archives of family medicine·1994

Area of Science:

  • Plant Physiology
  • Ion Transport
  • Plant Cell Biology

Background:

  • Understanding ion transport mechanisms is crucial for plant physiology.
  • Sodium (Na+) influx in plant roots is influenced by various factors, including membrane potential and other ions.
  • Corn (Zea mays L.) roots are a model system for studying nutrient uptake.

Purpose of the Study:

  • To investigate the effects of hyperpolarization, depolarization, potassium (K+), and calcium (Ca2+) on Na+ influx in corn root segments.
  • To differentiate between energy-dependent and independent Na+ transport pathways.
  • To elucidate the role of K+ and Ca2+ in regulating Na+ influx.

Main Methods:

  • Utilized radioactive tracer (22Na+) to measure Na+ influx in freshly excised and washed corn root segments.
  • Manipulated membrane potential using fusicoccin (hyperpolarization) and uncoupler (depolarization).
  • Assessed the impact of varying concentrations of K+ and Ca2+ on Na+ influx.

Main Results:

  • In freshly excised roots, Ca2+ significantly suppressed Na+ influx, while K+ and membrane potential changes had no effect.
  • Washed roots showed increased Na+ influx, sensitive to both hyperpolarization and depolarization.
  • This energy-linked influx was abolished by K+, suggesting involvement of the K+ carrier, while Ca2+ remained inhibitory.

Conclusions:

  • Na+ influx in corn roots involves both a K+-insensitive component (potentially voltage-sensitive or electroneutral exchange) and a K+-sensitive, energy-dependent component.
  • Ca2+ acts as a potent inhibitor of Na+ influx, irrespective of the root tissue's condition or membrane potential.
  • The findings highlight the complex regulation of Na+ transport in plants, involving ion interactions and energy-dependent processes.

Related Experiment Videos