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Related Experiment Videos

Chloroplast-Diphenyl Ether Interactions II.

S H Wettlaufer1, R Alscher, C Strick

  • 1Boyce Thompson Institute, Tower Road, Ithaca, New York 14853.

Plant Physiology
|June 1, 1985
PubMed
Summary
This summary is machine-generated.

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Acifluorfen herbicide disrupts photosynthesis by affecting chloroplast functions and inactivating key enzymes like fructose 1,6-bisphosphatase. This herbicide impacts stromal functions, influencing CO(2)-dependent O(2) evolution.

Area of Science:

  • Plant biochemistry
  • Photosynthesis research
  • Herbicide toxicology

Background:

  • Acifluorfen is a diphenyl ether herbicide impacting plant photosynthesis.
  • Its precise inhibitory mechanisms within chloroplasts require further elucidation.

Purpose of the Study:

  • To investigate the effects of acifluorfen on spinach chloroplast functions.
  • To identify the specific enzymes and pathways affected by acifluorfen.

Main Methods:

  • Studied acifluorfen's impact on CO(2)-dependent O(2) evolution in spinach chloroplasts.
  • Investigated acifluorfen penetration into chloroplast stroma.
  • Assessed inactivation of fructose 1,6-bisphosphatase and glyceraldehyde-3-phosphate dehydrogenase (NADP).

Main Results:

Related Experiment Videos

  • Acifluorfen inhibits photosynthetic functions requiring a functional chloroplast envelope and affects stromal functions.
  • It increases sensitivity of O(2) evolution to inorganic phosphate without directly impacting the phosphate translocator.
  • Acifluorfen penetrates the stroma independently of light and inactivates fructose 1,6-bisphosphatase and glyceraldehyde-3-phosphate dehydrogenase (NADP).

Conclusions:

  • Acifluorfen triggers an oxygen-dependent inactivation pathway for key photosynthetic enzymes.
  • This pathway involves thioredoxin and ferredoxin-thioredoxin reductase, highlighting a novel herbicidal mechanism.