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Linear sucrose transport in protoplasts from developing soybean cotyledons.

¹Central Research and Development Department, E. I. du Pont de Nemours and Company, Experimental Station, Wilmington, Delaware 19898.

Plant Physiology

|July 1, 1985

Summary

Soybean cells exhibit linear sucrose uptake, distinct from saturable processes. Proton/sucrose cotransport appears to drive this linear uptake, suggesting a dual mechanism for sucrose transport in these cells.

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Area of Science:

Plant Physiology
Molecular Biology
Biochemistry

Background:

Previous research indicated saturable and simple diffusion mechanisms for sucrose uptake in soybean cotyledon cells.
A proton/sucrose cotransport mechanism was hypothesized for saturable sucrose uptake.

Purpose of the Study:

To investigate the linear sucrose uptake mechanism in soybean cotyledon cells.
To determine if proton/sucrose cotransport is involved in the linear, nonsaturable uptake of sucrose.

Main Methods:

Isolated soybean cotyledon protoplasts were treated with p-trifluoromethoxy-carbonylcyanide phenylhydrazone (FCCP) or p-chloromecuribenzenesulfonic acid to inhibit saturable uptake.
The effects of external pH, fusicoccin, and FCCP on linear sucrose uptake were analyzed.
The ratio of proton to sucrose influx was measured.

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Main Results:

Linear sucrose uptake decreased with increasing external pH.
Fusicoccin stimulated linear sucrose uptake, while FCCP inhibited it.
The proton to sucrose influx ratio was approximately one for both saturable and nondiffusive linear uptakes.

Conclusions:

Proton/sucrose cotransport is implicated in the nondiffusive linear sucrose uptake pathway.
Soybean cotyledon cells utilize both saturable and linear cotransport mechanisms for sucrose uptake.