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Plunge Freezing: A Tool for the Ultrastructural and Immunolocalization Studies of Suspension Cells in Transmission Electron Microscopy
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A novel method of natural cryoprotection : intracellular glass formation in deeply frozen populus

A G Hirsh1, R J Williams, H T Meryman

  • 1American Red Cross, Biomedical Research Laboratories, Bethesda, Maryland 20814.

Plant Physiology
|September 1, 1985
PubMed

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Cryo-electron Microscopy01:28

Cryo-electron Microscopy

Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...