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Updated: Aug 8, 2026

Efficient Polyethylene Glycol (PEG) Mediated Transformation of the Moss Physcomitrella patens
Published on: April 19, 2011
A simple procedure to overcome polyethelene glycol toxicity on whole plants
1Division of Soils and Water, Agricultural Research Organization, The Volcani Center, Bet-Dagan 50250, Israel.
Abstract:
A procedure is described that can be used to minimize toxic effects of polyethylene glycol (PEG) to plants. The procedure is based on recycling nutrient solutions containing PEG-6000 through two plant cultures. Tomato plants grown in -0.3 megapascals PEG solutions used after two growth cycles exhibited minimal toxic effects. Long-term responses like dry matter production and chlorophyll content as well as short-term responses like CO(2) fixation rates and leaf conductance were severely inhibited by fresh PEG-6000 and only slightly reduced by recycled PEG-6000. Complete osmotic adjustment was obtained with tomatoes grown in recycled but not in fresh PEG solutions.

