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Phycomyces: fine structure analysis of the growing zone
R I Gamow1, G A Geer, B Bottger
1Department of Chemical Engineering, University of Colorado, Boulder, Colorado 80309.
Plant Physiology
|March 1, 1986
Summary
This study analyzed the Phycomyces sporangiophore growing zone (GZ), confirming its non-uniformity. Elongation and rotation rates vary independently within the GZ, challenging previous models.
Area of Science:
- Mycology
- Cell Biology
- Biophysics
Background:
- The growing zone (GZ) of Phycomyces sporangiophores is crucial for cell elongation.
- Previous studies suggested a uniform GZ with counterclockwise rotation.
Purpose of the Study:
- To perform a fine structure analysis of the Phycomyces sporangiophore GZ.
- To investigate the uniformity and rotational dynamics within the GZ.
- To evaluate the fibril reorientation model.
Main Methods:
- Utilized a computer-video digitizer and recorder for high-resolution analysis.
- Simultaneously tracked the trajectory of two independent particles within and above the GZ.
- Measured rates of rotation and elongation as a function of position in the GZ.
Main Results:
- Confirmed that the GZ is not uniform, consistent with Cohen and Delbrück (1958).
- Did not confirm the existence of counterclockwise rotation in mature sporangiophores.
- Observed independent and non-linear changes in rotation and elongation rates within the GZ.
Conclusions:
- The non-uniformity of the GZ is a key factor in Phycomyces growth.
- The observed dynamics challenge previous assumptions about GZ structure and rotation.
- Findings support the importance of the fibril reorientation model in explaining sporangiophore growth.