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Related Experiment Videos

Daptomycin disrupts membrane potential in growing Staphylococcus aureus.

W E Alborn1, N E Allen, D A Preston

  • 1Infectious Disease Research, Lilly Research Laboratories, Eli Lilly and Company, Indianapolis, Indiana 46285.

Antimicrobial Agents and Chemotherapy
|November 11, 1991
PubMed
Summary

Daptomycin disrupts the membrane potential in Staphylococcus aureus, a key factor in its antibacterial activity. This calcium-dependent effect on membrane energization explains its inhibitory actions.

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Area of Science:

  • Microbiology
  • Biochemistry
  • Molecular Biology

Background:

  • Daptomycin is a cyclic lipopeptide antibiotic effective against Gram-positive bacteria.
  • Its precise mechanism of action, particularly its effect on bacterial energetics, requires further elucidation.

Purpose of the Study:

  • To investigate the impact of daptomycin on the membrane potential (delta psi) and proton motive force in Staphylococcus aureus.
  • To determine the role of calcium in daptomycin's membrane-disrupting effects.
  • To correlate membrane potential dissipation with daptomycin's bactericidal activity.

Main Methods:

  • Utilized membrane potential-sensitive dyes to measure delta psi in Staphylococcus aureus.
  • Assessed the chemical gradient (delta pH) across the bacterial membrane.

Related Experiment Videos

  • Correlated changes in membrane energization with bacterial growth inhibition and viability assays.

    • Main Results:

    • Daptomycin induced a rapid and significant dissipation of the membrane potential (delta psi) in Staphylococcus aureus.
    • This effect was dependent on the presence of calcium ions.
    • Daptomycin did not significantly alter the delta pH across the bacterial membrane.
    • The observed dissipation of delta psi correlated directly with the bactericidal activity of daptomycin.

    Conclusions:

    • Daptomycin's primary mechanism involves the disruption of the bacterial membrane potential.
    • Calcium is essential for daptomycin's ability to dissipate delta psi.
    • The loss of membrane potential is a critical event leading to daptomycin's bactericidal effect against Staphylococcus aureus.