Related Experiment Video
Updated: Aug 8, 2026

Monitoring Plant Hormones During Stress Responses
Published on: June 15, 2009
A highly sensitive method for quantitative determination of abscisic Acid
C H Michler1, R D Lineberger, G W Chism
1Department of Horticulture, The Ohio Agricultural Research and Development Center, The Ohio State University, Columbus, Ohio 43210.
Abstract:
An abscisic acid derivative was formed by reaction with pentafluorobenzyl bromide which allowed highly sensitive detection by gas-liquid chromatography with electron capture detection. In comparison to the methyl ester derivative, the pentafluorobenzyl derivative of abscisic acid was four times more sensitive to electron capture detection and was stable at room temperature in the presence of ultraviolet light. Derivatization was rapid and the molecular weight of the new compound was confirmed by gas-liquid chromatography-mass spectrometry.
More Related Videos
10:13Using Capillary Electrophoresis to Quantify Organic Acids from Plant Tissue: A Test Case Examining Coffea arabica Seeds
Published on: November 12, 2016
10:22Improved UPLC-UV Method for the Quantification of Vitamin C in Lettuce Varieties (Lactuca sativa L.) and Crop Wild Relatives (Lactuca spp.)
Published on: June 30, 2020
Related Concept Videos
Atomic Absorption Spectroscopy: Lab
Solutions containing organic solvents, such as low-molecular-mass alcohols, esters, or ketones, enhance absorbances by increasing nebulizer...
Atomic Absorption Spectroscopy: Overview
When irradiated by EMR of a particular wavelength, these...
Atomic Absorption Spectroscopy: Atomization Methods
Atomic Absorption Spectroscopy: Instrumentation
The atomizer used in AAS can be either a flame atomizer or an...