Related Experiment Video
Updated: Aug 8, 2026

A Flexible Low Cost Hydroponic System for Assessing Plant Responses to Small Molecules in Sterile Conditions
Published on: August 25, 2018
Subcellular Metabolite Levels in Spinach Leaves : Regulation of Sucrose Synthesis during Diurnal Alterations in
R Gerhardt1, M Stitt, H W Heldt
1Institut fur Biochemie der Pflanze, Untere Karspule 2, 3400 Gottingen, Federal Republic of Germany.
Abstract:
The alterations of subcellular metabolite levels during the day in spinach leaves have been investigated using nonaqueous density gradient centrifugation to separate chloroplasts, cytosol, and vacuole. The results provide direct evidence for the role of sucrose phosphate synthase and cytosolic fructose 1,6-bisphosphatase in regulating sucrose synthesis in leaves and also show that the phosphate translocator is kinetically limiting in vivo.
Related Concept Videos
Overview of Metabolism
Plant Metabolism
Sunlight, the primary source of energy in plants, is first absorbed by the chlorophyll pigments present in their leaves. Plants then use this energy to carry out photosynthesis, where water is oxidized into oxygen and carbon dioxide...
The Calvin Benson Cycle
Phloem and Sugar Transport
Regulation of Transpiration by Stomata
Adaptations that Reduce Water Loss
Key Elements for Plant Nutrition
