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Related Concept Videos

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The Citric Acid Cycle

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Related Experiment Video

Updated: Jun 25, 2026

Caffeine Extraction, Enzymatic Activity and Gene Expression of Caffeine Synthase from Plant Cell Suspensions
09:11

Caffeine Extraction, Enzymatic Activity and Gene Expression of Caffeine Synthase from Plant Cell Suspensions

Published on: October 2, 2018

Enzymic synthesis of caffeoylglucaric Acid from chlorogenic Acid and glucaric Acid by a protein preparation from

D Strack1, W Gross, V Wray

  • 1Botanisches Institut der Universität zu Köln, Gyrhofstrasse 15, D-5000 Köln 41, Federal Republic of Germany.

Plant Physiology
|March 1, 1987
PubMed
Summary
This summary is machine-generated.

Tomato cotyledons metabolize phenylpropanes, forming chlorogenic and caffeoylglucaric acids. An enzyme in tomato cotyledons catalyzes caffeoylglucaric acid formation from chlorogenic acid and glucaric acid.

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13C6-Glucose Labeling Associated with LC-MS: Identification of Plant Primary Organs in Secondary Metabolite Synthesis

Published on: March 22, 2024

Area of Science:

  • Plant biochemistry
  • Metabolomics
  • Enzymology

Background:

  • Phenylpropane metabolism is crucial for plant development and defense.
  • Hydroxycinnamic acids are key intermediates in phenylpropane pathways.
  • Understanding these pathways in tomato (Lycopersicon esculentum Mill) can reveal novel biochemical processes.

Purpose of the Study:

  • To investigate the phenylpropane metabolism in tomato cotyledons.
  • To identify the major hydroxycinnamic acid conjugates.
  • To elucidate the enzymatic mechanisms involved in their formation.

Main Methods:

  • High-performance liquid chromatography (HPLC) for component analysis.
  • Quantitative analysis to establish precursor-product relationships.
  • Enzyme assays using protein preparations from tomato cotyledons.

Main Results:

  • Chlorogenic acid (5-O-caffeoylquinic acid) and caffeoylglucaric acid were identified as major hydroxycinnamic-acid conjugates.
  • A precursor-product relationship was established between chlorogenic and caffeoylglucaric acids.
  • Enzyme activity was detected, catalyzing caffeoylglucaric acid formation from chlorogenic acid and glucaric acid.

Conclusions:

  • Tomato cotyledons possess a distinct phenylpropane metabolic pathway.
  • Enzymatic conversion of chlorogenic acid to caffeoylglucaric acid occurs.
  • The identified enzyme activity contributes to the biosynthesis of glucaric acid conjugates in plants.