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Altered Gene Expression during Auxin-Induced Root Development from Excised Mung Bean Seedlings
R S Dhindsa1, G Dong, L Lalonde
1Center for Plant Molecular Biology, McGill University, 1205 Avenue Docteur Penfield, Montreal, Quebec, H3A 1B1, Canada.
Plant Physiology
|August 1, 1987
Summary
Auxin triggers specific protein synthesis, preceding root development in mung bean seedlings. This indicates that altered gene expression is crucial for initiating root formation.
Area of Science:
- Plant Biology
- Molecular Biology
- Biochemistry
Background:
- Plant hormones, like auxin, play critical roles in plant development.
- Root development is a complex process involving hormonal regulation and gene expression changes.
Purpose of the Study:
- To investigate the changes in protein synthesis during auxin-induced root development in mung bean seedlings.
- To identify specific proteins synthesized in response to auxin treatment and their role in root initiation.
Main Methods:
- Excised mung bean seedlings were treated with auxin or antiauxin.
- Protein synthesis patterns were analyzed using two-dimensional polyacrylamide gel electrophoresis.
- Messenger RNA (mRNA) populations were studied using in vitro translation assays.
Main Results:
- Auxin treatment induced the synthesis of specific low molecular weight, high pI proteins.
- These auxin-induced proteins appeared transiently, preceding visible root development.
- Antiauxin treatment inhibited auxin-induced rooting and the synthesis of these specific proteins.
- In vitro translation of mRNA from auxin-treated tissue confirmed the synthesis of these proteins.
Conclusions:
- Auxin-induced root formation involves significant alterations in gene expression.
- The identified auxin-induced proteins likely play a regulatory role in the initiation of root development.
